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M0529

Sigma-Aldrich

Murashige and Skoog Basal Salt Micronutrient Solution

10 ×, liquid, suitable for plant cell culture

NACRES:
NA.72

sterility

sterile-filtered

Quality Level

form

liquid

concentration

10 ×

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

shipped in

ambient

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M0529) contain is a 10x concentrate of the micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Liam Walker et al.
The Plant cell, 29(10), 2393-2412 (2017-09-13)
Shaping of root architecture is a quintessential developmental response that involves the concerted action of many different cell types, is highly dynamic, and underpins root plasticity. To determine to what extent the environmental regulation of lateral root development is a...
Yuan Su et al.
The Plant journal : for cell and molecular biology, 94(2), 315-326 (2018-02-14)
Phosphate (Pi) deficiency in soils is a major limiting factor for plant growth. In response to Pi deprivation, one prominent metabolic adaptation in plants is the decrease in membrane phospholipids that consume approximately one-third cellular Pi. The level of two...
Adrian N Dauphinee et al.
Plant physiology, 181(3), 855-866 (2019-09-07)
Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield....
Keiko Midorikawa et al.
Scientific reports, 9(1), 271-271 (2019-01-24)
Non-viral gene carriers have been extensively investigated as alternatives to viral vectors for gene delivery systems into animal and plant cells. A non-viral gene carrier containing a cell-penetrating peptide and a cationic sequence was previously developed for use in intact...
Xiao Huang et al.
The Plant cell, 31(12), 2973-2995 (2019-10-17)
Under nutrient and energy-limiting conditions, plants up-regulate sophisticated catabolic pathways such as autophagy to remobilize nutrients and restore energy homeostasis. Autophagic flux is tightly regulated under these circumstances through the AuTophaGy-related1 (ATG1) kinase complex, which relays upstream nutrient and energy...

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