Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution

MDL number:

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


F12B, monoclonal


buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

mouse, rat, human


immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
immunoprecipitation (IP): 2 μg using 1 mg protein lysate
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1 μg/mL





UniProt accession no.

shipped in

wet ice

storage temp.


Gene Information

human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)

General description

Myogenin belongs to the basic helix-loop-helix (bHLH) superfamily.
Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.


rat recombinant myogenin (amino acids 30-224).


Monoclonal Anti-Myogenin antibody produced in mouse has been used in immunohistochemistry, immunofluorescence and western blotting.

Biochem/physiol Actions

Myogenin acts as a biomarker in rhabdomyosarcoma (RMS). It has a role in developmental processes, such as hematopoiesis, neurogenesis, myogenesis, tumor cell invasion and metastasis.
Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Neuraminidase-1 mediates skeletal muscle regeneration
de Carvalho Neves J, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1852(9), 1755-1764 (2015)
A Pertille et al.
Brazilian journal of biology = Revista brasleira de biologia, 77(1), 83-91 (2016-07-08)
The aim of this study was to analyze muscle regeneration after cryoinjury in the tibialis anterior muscle of young rats that were malnourished and then recovered. Forty Wistar rats were divided into a nourished group that received a normal protein...
Dysregulated Myogenesis in Rhabdomyosarcoma
Current Topics in Developmental Biology, 126(9), 285-297 (2018)
W Glenn McCluggage et al.
Histopathology, 63(4), 545-550 (2013-08-16)
Myogenin (myf4) is a nuclear transcription factor that is considered to be a sensitive and highly specific marker for skeletal muscle differentiation. Following the identification of focal strong nuclear staining with myogenin in two fibroepithelial polyps of the lower female...

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