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M6899

Sigma-Aldrich

Murashige and Skoog Basal Salts with minimal organics

powder, suitable for plant cell culture

Synonym(s):
MSMO
NACRES:
NA.72

form

powder

Quality Level

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.

Pictograms

Flame over circleExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 2

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Organic growth factor requirements of tobacco tissue cultures.
Linsmaier, E.M. and Skoog, F.
Physiologia Plantarum, 18, 100-127 (1965)
Okhee Choi et al.
Plant physiology, 146(2), 657-668 (2007-12-07)
Pseudomonas fluorescens B16 is a plant growth-promoting rhizobacterium. To determine the factors involved in plant growth promotion by this organism, we mutagenized wild-type strain B16 using OmegaKm elements and isolated one mutant, K818, which is defective in plant growth promotion...
Li Wang et al.
Planta, 249(4), 1107-1118 (2018-12-16)
In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta...
Alexandra Lešková et al.
PloS one, 15(8), e0237448-e0237448 (2020-08-14)
We established that Endosidin2 (ES2) affected the trafficking routes of both newly synthesized and endocytic pools of PIN-FORMED2 (PIN2) in Arabidopsis root epidermal cells. PIN2 populations accumulated in separated patches, which gradually merged into large and compact ES2 aggregates (ES2As)....
Jeremie Bazin et al.
PLoS pathogens, 16(4), e1008401-e1008401 (2020-04-18)
Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism of gene regulation in environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is mediated by mitogen-activated protein kinases and the majority...

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