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M7024

Sigma-Aldrich

Murashige and Skoog Shoot Multiplication Medium A

powder, suitable for plant cell culture

Synonym(s):
MSMA

form

powder

Quality Level

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

shipped in

ambient

storage temp.

2-8°C

Application

Murashige and Skoog Shoot Multiplication Medium A is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP as described by Huang and Murashige (1976) TCA Manual.

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M7024 is a variant media) with macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP.

Pictograms

Flame over circle

Signal Word

Warning

Hazard Statements

Hazard Classifications

Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

R H Smith et al.
In vitro, 13(5), 329-334 (1977-05-01)
Defined in vitro conditions for callus initiation by Gossypium arboreum L. were determined, and different tissues were evaluated as explant sources, Environmental conditions tested included light versus dark, and low light versus high light. Different nutrient media as well as...
Akasaka et al.
Plant science : an international journal of experimental plant biology, 156(2), 169-175 (2000-08-11)
Bud primordia were induced from leaf segments, which were harvested from young seedlings of Spanish type peanut (Arachis hypogaea L. cv. Chico), on 0.8% agar-solidified medium containing Murashige and Skoog (MS) basal salts supplemented with B5 vitamins, 1 mg/l NAA...
Plant tissue culture media: major constituents, their preparation and some applications.
Huang L, Murashige T.
Tissue Antigens, 3, 539-548 (1976)
Regulation of lycopene formation in cell suspension culture of VFNT tomato (Lycopersicon esculentum) by CPTA, growth regulators, sucrose, and temperature
Robertson GE, Mahoney NE
Journal of Experimental Botany, 46, 667-673 (1995)
Nor Azlina Hasbullah et al.
Pakistan journal of biological sciences : PJBS, 11(11), 1449-1454 (2008-09-27)
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds...

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