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M9274

Sigma-Aldrich

Murashige and Skoog Basal Medium

suitable for plant cell culture, with sucrose and agar

Synonym(s):
MS Basal Medium
MDL number:
NACRES:
NA.72

Quality Level

form

powder

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used for culturing Arabidopsis transgenic seeds for cotyledon development, pearl millet (Pennisetum glaucum), and Rosmarinus ofcinalis L. for callus induction

Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Pictograms

Flame over circleExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?

    This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.

  6. How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?

    M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.

  7. How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?

    This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antoine Fort et al.
The New phytologist, 209(2), 590-599 (2015-09-24)
Heterosis is the phenomenon whereby hybrid offspring of genetically divergent parents display superior characteristics compared with their parents. Although hybridity and polyploidy can influence heterosis in hybrid plants, the differential contributions of hybridity vs polyploidy to heterosis effects remain unknown....
Baba Ngom et al.
Plant signaling & behavior, 12(9), e1356967-e1356967 (2017-08-02)
Cytosine DNA methylation is an epigenetic regulatory system used by plants to control gene expression. Methylation pattern always changes after abiotic stresses, pathogens and pest infections or after a treatment with salicylic acid (SA). The latter is a key player...
Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)
Coskun Y, et al.
Plant Cell, Tissue and Organ Culture, 138(1), 89-95 (2019)
Laure Audonnet et al.
Gene expression patterns : GEP, 25-26, 1-7 (2017-04-13)
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work...
Smita P Chavan et al.
Applied microbiology and biotechnology, 89(6), 1701-1707 (2010-12-02)
The present study examined the effects of plant growth hormones, incubation period, biotic (Trametes versicolor, Mucor sp., Penicillium notatum, Rhizopus stolonifer, and Fusarium oxysporum) and abiotic (NaCl, MgSO(4), FeSO(4), ZnSO(4), and FeCl(3)) elicitors on cell growth and α-tocopherol and pigment...

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