MilliporeSigma
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MAK110

Sigma-Aldrich

MTP Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonym(s):
Microsomal triglyceride transfer protein activity assay kit, MTTP Activity Assay Kit
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

2-8°C

Gene Information

human ... MTTP(4547)

Related Categories

General description

Microsomal triglyceride transfer protein (MTP), a membrane-bound protein present in the liver, plays an important role in the assembly and secretion of very low density lipoprotein (VLDL). MTP is also necessary for assembly and secretion of chylomicrons by the intestine. This protein facilitates the transfer of triglyceride (TG), cholesteryl ester (CE) and phosphatidylcholine (PC) between phospholipid surfaces. Mutation in the MTP gene leads to abetalipoproteinemia.

Application

MTP Activity Assay Kit has been used to measure the activity of microsomal triglyceride transfer protein (MTP).

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring microsomal [triglyceride] transfer protein (MTP) activity in cell lysates or tissue homogenates, or for screening MTP activity inhibitors.

Principle

The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate. This kit includes proprietary substrates that enable the detection of MTP-mediated transfer of neutral lipid. The transfer activity results in an increase in fluorescence intensity (λEx=465 nm/λEm=535 nm).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

J Massart et al.
British journal of pharmacology, 165(5), 1361-1374 (2011-07-12)
Pentoxifylline is in clinical trials for non-alcoholic fatty liver disease and diabetic nephropathy. Metabolic and hepatic effects of pentoxifylline were assessed in a murine model of obesity and type 2 diabetes. Pentoxifylline (100 mg·kg(-1) ·day(-1)) was administered for 4 days
Thi Thu Trang Tran et al.
The Journal of biological chemistry, 291(31), 16328-16338 (2016-06-04)
The worldwide prevalence of metabolic diseases is increasing, and there are global recommendations to limit consumption of certain nutrients, especially saturated lipids. Insulin resistance, a common trait occurring in obesity and type 2 diabetes, is associated with intestinal lipoprotein overproduction.
Microsomal triglyceride transfer protein specificity of lipid binding and transport.
Jamil H, et al.
The Journal of Biological Chemistry, 270(12), 6549-6554 (1995)
Short-term palmitate supply impairs intestinal insulin signaling via ceramide production.
Tran T T T, et al.
The Journal of Biological Chemistry, M115-M115 (2016)
Qinyuan Ye et al.
Hepatology research : the official journal of the Japan Society of Hepatology, 43(10), 1115-1123 (2013-02-21)
To investigate the role of Cytochrome P4502E1 in sensitizing Kupffer cells to lipopolysaccharide (LPS)-mediated inflammation after ethanol induction. Sprague-Dawley rats were fed a liquid ethanol diet, control diet or ethanol diet supplemented with CYP2E1 inhibitor, chlormethiazole (CMZ), for 4 weeks. Hepatic

Protocols

LCAT Activity Assay Kit

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of LCAT.

MTP Activity Assay Kit Protocol

The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate.

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