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MAK326

Sigma-Aldrich

Indole Assay Kit

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

2-8°C

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Indole Assay Kit sufficient for 100 colorimetric tests

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Alanine Assay Kit sufficient for 100 colorimetric or fluorometric tests

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Alanine Assay Kit

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric, fluorometric

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Indole is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole.

Suitability

Suitable for indole determination in biological samples (e.g. indole produced by indole positive bacteria).

Principle

The indole assay kit is based on a modified version of Ehlrich′s and Kovac′s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample. Linear detection range from 3 to 100 μM indole in 96-well plate assay.

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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