MD0100

Sigma-Aldrich

MGMT Assay Kit

sufficient for 100 tests

Synonym(s):
O6-Methylguanine-DNA Methyltransferase Assay Kit
Pricing and availability is not currently available.

usage

sufficient for 100 tests

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MGMT(4255)

Application

MGMT (O6-Methylguanine-DNA methyltransferase) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine lesions, primarily O6-methyl-guanine from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation.
The MGMT assay kit is based on the formation of a new restriction site in a 23-mer 32P labeled ds-oligonucleotide after removal of the O6-methyl-guanine lesion. Cleavage of the new restriction site results in two smaller oligonucleotides that can be resolved on a denaturing PAGE gel.

Features and Benefits

The kit can be used for:
  • Screening for MGMT inhibitor.
  • Detection of MGMT activity in cell lines.

Kit Components Only

Product No.
Description

  • MGMT substrate O6-methylguanine strand 1 mL/vial

  • MGMT substrate complementary strand 1 mL/vial

  • Reaction buffer 10X 1.5 mL

  • Stop solution 1 mL

  • Methylguanine DNA methyltransferase (MGMT) 10 μg

  • O6Benzylguanine solution 50 μL

  • Spin column-10 2 U

Hazard Codes

T

Risk Statement

61-20/21-36

Safety Statement

53.1-45

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
W K Hansen et al.
The Journal of pharmacology and experimental therapeutics, 295(1), 1-9 (2000-09-19)
The area of mammalian DNA repair and its relationship to cancer and therapeutic approaches is rapidly growing, both through the studies of basic mechanisms and in the use of this knowledge for translational applications. We have attempted to briefly and...
Geoffrey P Margison et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 24(3), 255-266 (2002-03-14)
The DNA in human cells is continuously undergoing damage as consequences of both endogenous processes and exposure to exogenous agents. The resulting structural changes can be repaired by a number of systems that function to preserve genome integrity. Most pathways...
M Sassanfar et al.
The Journal of biological chemistry, 266(5), 2767-2771 (1991-02-15)
The suicidal inactivation mechanism of DNA repair methyltransferases (MTases) was exploited to measure the relative efficiencies with which the Escherichia coli, human, and Saccharomyces cerevisiae DNA MTases repair O6-methylguanine (O6MeG) and O4-methylthymine (O4MeT), two of the DNA lesions produced by...
L P Encell et al.
Biochemistry, 38(37), 12097-12103 (1999-10-03)
Human O(6)-alkylguanine-DNA alkyltransferase (MGMT) repairs potentially cytotoxic and mutagenic alkylation damage at the O(6)-position of guanine and the O(4)-position of thymine in DNA. We have used random sequence mutagenesis and functional complementation to obtain human MGMT mutants that are resistant...

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