Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.
Features and Benefits
ELISA based format
Procedure completes in 4 easy to follow steps
All reagents supplied including control methylated DNA
Detection limit is 5 ng of fully methylated DNA
Input DNA may be as low as 10-200 ng
Procedure completes in 3.5 hours
96 well format allows option of studying single samples or high-throughput studies
Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Imprint is a registered trademark of Sigma-Aldrich Co. LLC