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MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

NACRES:
NA.32

packaging

pkg of 1 kit

Quality Level

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

application(s)

cell analysis: suitable
detection: suitable
flow cytometry: suitable

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Related Categories

General description

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Application

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description

  • Diluent C 10 mL

  • PKH67 Cell Linker in ethanol .1 mL

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

55.4 °F - closed cup

Flash Point(C)

13 °C - closed cup

Certificate of Analysis

Certificate of Origin

Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization
Huang C, et al.
Molecular Medicine Reports, 18(1), 509-515 (2018)
A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures
Tseng HY, et al.
Biopreservation and Biobanking, 9(4), 355-362 (2011)
Simone Moertl et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component...
Haiyang Xu et al.
Stem cell research & therapy, 10(1), 381-381 (2019-12-18)
Mesenchymal stem cells (MSCs) play a significant role in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the effects of exosomal miR-133b derived from MSCs on glioma...
Hongbin Wang et al.
Molecular therapy. Nucleic acids, 19, 654-667 (2020-01-20)
Recently, novel mechanisms underlying the pro-tumorigenic effects of cancer-associated fibroblasts (CAFs) have been identified in several cancers, including breast cancer. CAFs can secrete exosomes that are loaded with proteins, lipids, and RNAs to affect tumor microenvironment. Herein, we identify CAF-derived...

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