MITOISO1

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

EC Number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)

shelf life

2 yr

shipped in

dry ice

storage temp.

−20°C

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Application

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
  • for the isolation of mitochondria from glomeruli and heart
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

Features and Benefits

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Kit Components Also Available Separately

Product No.
Description
SDS

  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Target Organs

Respiratory system

Hazard Codes

Xn

Risk Statement

36/37/38-42

Safety Statement

22-24-26-36/37

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?

    It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.

  3. Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?

    The extraction buffers supplied with this kit are only available with the kit.

  4. When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?

    If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

  5. How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?

    The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.

  6. Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?

    Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?

    Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

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