LookOut® mycoplasma qPCR detection kit

EC Number:


sufficient for 25 reactions


Not suitable for clinical diagnostic use.


PCR: suitable
detection: suitable
microbiology: suitable

storage temp.


General description

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 16S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit has been tested and optimized for use with JumpStartä Taq DNA Polymerase, Catalog Number D9307. It is highly recommended that this product be used with the kit. Use of other Taq polymerase products may require reaction optimization to achieve proper results.


qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Legal Information

LookOut is a registered trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Renxu Chang et al.
Nature communications, 9(1), 3486-3486 (2018-08-30)
Loss of WW domain-containing oxidoreductase (Wwox) expression has been observed in breast cancer (BC). However, its regulatory effects are largely unknown, especially in triple-negative breast cancer (TNBC). Herein, gene expression profiling revealed that JAK/STAT3 pathway was one of the most...
Ji Hye Yang et al.
Life science alliance, 3(7) (2020-06-04)
Despite the importance of mitochondrial fatty acid oxidation (FAO) in cancer metabolism, the biological mechanisms responsible for the FAO in cancer and therapeutic intervention based on catabolic metabolism are not well defined. In this study, we observe that Snail (SNAI1)...
Stella D'Oronzo et al.
International journal of molecular sciences, 21(17) (2020-08-28)
DEAD-Box Helicase 4 (Ddx4)+ ovarian stem cells are able to differentiate into several cell types under appropriate stimuli. Ddx4 expression has been correlated with poor prognosis of serous ovarian cancer (OC), while the potential role of Ddx4+ cells in non-serous...

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