N1533

Sigma-Aldrich

Nitric Oxide Synthase, Endothelial bovine

recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution

Synonym(s):
NOS III, ecNOS
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous solution

specific activity

≥0.3 units/mg protein

mol wt

135 (kDa/subunit, homodimer)

shipped in

dry ice

storage temp.

−70°C

Gene Information

cow ... NOS3(287024)

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine and is constituitively expressed in endothelial cells (eNOS) and brain (nNOS).
Suitable for measuring NOS activity in the presence of exogenously added co-factors and/or inhibitors.

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 5 mM CHAPS, and 100 μM DTT.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Silviya R Stateva et al.
PloS one, 10(4), e0120798-e0120798 (2015-04-02)
Calmodulin (CaM) phosphorylated at different serine/threonine and tyrosine residues is known to exert differential regulatory effects on a variety of CaM-binding enzymes as compared to non-phosphorylated CaM. In this report we describe the preparation and characterization of a series of...
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 50(1), 84-89 (2012-01-17)
The cholesterol ozonolysis products secosterol-A and its aldolization product secosterol-B were recently detected in human atherosclerotic tissues and brain specimens, and have been postulated to play pivotal roles in the pathogenesis of atherosclerosis and neurodegenerative diseases. We examined several oxidized...
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 46(2), 177-185 (2010-03-11)
N(omega)-Carboxymethyl-arginine (CMA), N(omega)-carboxyethyl-arginine (CEA) and N(delta)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) have been identified as L-arginine-derived advanced glycation end products (AGEs) formed by non-enzymatic reactions between reducing sugars such as glucose and amino groups in proteins. These AGEs are structurally analogous to endogenous inhibitors...
Yunzhou Dong et al.
Journal of cellular and molecular medicine, 13(9A), 2899-2910 (2008-07-16)
Oxidation and glycation of low-density lipoprotein (LDL) promote vascular injury in diabetes; however, the mechanisms underlying this effect remain poorly defined. The present study was conducted to determine the effects of 'heavily oxidized' glycated LDL (HOG-LDL) on endothelial nitric oxide...
Emre Gazyakan et al.
Annals of plastic surgery, 84(6), 705-710 (2019-12-19)
Nitric oxide (NO) is a multifunctional signaling molecule involved in regulating vascular tone and tissue oxygenation. It is also an important cytoprotective agent against ischemia-reperfusion injury (IRI). Enhancing NO bioavailability via exogenous NO synthases (NOSs) and L-arginine promotes conversation to...

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