N3033

Sigma-Aldrich

Nitric Oxide Synthase, Neuronal from rat

≥95%, recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution

Synonym(s):
NOS I, bNOS, Neuronal Nitric Oxide Synthase, ncNOS
MDL number:
Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥95%

form

buffered aqueous solution

specific activity

≥200 units/mg protein

mol wt

150 (kDa/subunit, homodimer)

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Nos1(24598)

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.

RIDADR

NONH for all modes of transport

WGK Germany

1

Holger Engel et al.
Annals of plastic surgery, 79(4), e25-e29 (2014-04-03)
Nitric oxide (NO) is an important cytoprotective agent against ischemia and reperfusion injury (IRI). Enhancing NO bioavailability via exogen NO synthases (NOSs) and L-arginine promotes conversation to NO, circumventing the problem of nonfunctioning NOSs under hypoxic and acidic conditions. In...
Michał Bulc et al.
World journal of gastroenterology, 23(33), 6088-6099 (2017-10-04)
Influence of chronic hyperglycemia on chemical coding of enteric neurons in stomach using pig as a model for human diabetic complications. Ten pigs were divided into two groups: diabetic (D group, In the control group in the myenteric ganglia (MG)...
Darío E Iglesias et al.
Food & function, 10(5), 2528-2537 (2019-04-18)
In order to study the in vitro effect of flavan-3-ol (+)-catechin on the enzymatic activities of mitochondrial complex I and nitric oxide synthase (mtNOS), as well as the consequences on the membrane potential and H2O2 production rate, isolated mitochondria from...
Emilio Varea et al.
Frontiers in neuroscience, 5, 17-17 (2011-03-19)
Neuronal production persists during adulthood in the dentate gyrus and the olfactory bulb, where substantial numbers of immature neurons can be found. These cells can also be found in the paleocortex layer II of adult rodents, but in this case...
Emre Gazyakan et al.
Annals of plastic surgery, 84(6), 705-710 (2019-12-19)
Nitric oxide (NO) is a multifunctional signaling molecule involved in regulating vascular tone and tissue oxygenation. It is also an important cytoprotective agent against ischemia-reperfusion injury (IRI). Enhancing NO bioavailability via exogenous NO synthases (NOSs) and L-arginine promotes conversation to...

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