N7265

Sigma-Aldrich

Nitrate Reductase (NADPH) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Synonym(s):
NAD(P)H:Nitrate oxidoreductase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

specific activity

≥300 units/g solid

shipped in

wet ice

storage temp.

−20°C

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General description

Nitrate reductase (NADPH) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NADPH) is 180,000Daltons.

Packaging

2, 5 units in glass bottle
10 units in poly bottle

Biochem/physiol Actions

Nitrate Reductase (NADPH) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.

Caution

light and moisture sensitive

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

G Unden et al.
Molecular microbiology, 25(2), 205-210 (1997-07-01)
The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The...
M A de la Rosa et al.
European journal of biochemistry, 106(1), 249-256 (1980-05-01)
Assimilatory nitrate reductase [NAD(P)H] (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by a simple procedure that utilizes as the main step affinity chromatography on Blue-Sepharose. The best enzyme preparation has a specific activity of 61.25 units/mg protein....
T Schöndorf et al.
Plant physiology, 108(1), 203-210 (1995-05-01)
Differences in the amino acid sequence between the bispecific NAD(P)H-nitrate reductase of birch (Betula pendula Roth) and the monospecific NADH-nitrate reductases of a variety of other higher plants have been found at the dinucleotide-binding site in the FAD domain. To...
N Hall et al.
Microbiology (Reading, England), 146 ( Pt 6), 1399-1406 (2000-06-10)
Nitrate reductase (NaR) catalyses the reduction of nitrate to nitrite via a two-electron transfer. In fungi, the electron donor for NaR is NADPH whereas plants can have two enzymes, NADH:NaR and a bispecific NAD(P)H:NaR. PCR mutagenesis was employed to introduce...
J Miyazaki et al.
Molecular & general genetics : MGG, 228(3), 329-334 (1991-09-01)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from...

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