Documents

NOS1

Sigma-Aldrich

Nitric Oxide Synthase Detection Kit, Isotopic

sufficient for 50 assays

Synonym(s):
NOS Detection Kit
Pricing and availability is not currently available.

usage

sufficient for 50 assays

foreign activity

nuclease and protease, free

Application

Sigma′s Nitric Oxide Synthase Detection System is a simple and specific assay for the measurement of nitric oxide synthase (NOS) activity. Nitric oxide (NO), formed from the amino acid L-arginine via the action of the NOS enzyme, is a unique cell signaling molecule that functions as both an intracellular and an extracellular messenger. NO has been implicated in vasodilation, neurotransmission, cytotoxicity, and inflammation.1
This kit utilizes a highly specific and sensitive assay for the direct quantitative measurement of nitric oxide synthase activity. The assay measures the enzymatic conversion of 3H-arginine to 3H-citrulline and is the standard assay for measuring NOS activity in cell lysates, tissue homogenates and purified enzyme preparations. Activity is measured in the picomole range and a positive control is included.

Caution

Upon receipt, store Box 1 at −70 °C, Box 2 at room temperature.

Kit Components Only

Product No.
Description

  • Microcentrifuge tubes 50 ea

Kit Components Also Available Separately

Product No.
Description
SDS

  • Equilibrated Resin for NOS Detection System 5 mL

  • Spin columns and caps 50 ea

  • Stop Buffer for NOS Detection System 20 mL

Certificate of Analysis
Certificate of Origin
D J Stuehr
Annual review of pharmacology and toxicology, 37, 339-359 (1997-01-01)
Research on the biological roles of nitric oxide has revealed that it functions as an important signal and effector molecule in a variety of physiologic and pathologic settings. In animals, nitric oxide is synthesized enzymatically from L-arginine through the actions...
D A Geller et al.
Cancer metastasis reviews, 17(1), 7-23 (1998-04-17)
Nitric oxide (NO) is a potent biologic mediator with diverse physiologic and pathophysiologic roles. NO is produced from L-arginine by the family of nitric oxide synthase (NOS) enzymes, forming the free radical NO and citrulline as byproduct. Three distinct isoforms...
D S Bredt et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(22), 9030-9033 (1989-11-01)
Nitric oxide, which mediates influences of numerous neurotransmitters and modulators on vascular smooth muscle and leukocytes, can be formed in the brain from arginine by an enzymatic activity that stoichiometrically generates citrulline. We show that glutamate and related amino acids...
M A Marletta et al.
Current opinion in chemical biology, 2(5), 656-663 (1998-11-18)
The enzyme nitric oxide synthase catalyzes the oxidation of the amino acid L-arginine to L-citrulline and nitric oxide in an NADPH-dependent reaction. Nitric oxide plays a critical role in signal transduction pathways in the cardiovascular and nervous systems and is...
D S Bredt et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(2), 682-685 (1990-01-01)
Nitric oxide mediates vascular relaxing effects of endothelial cells, cytotoxic actions of macrophages and neutrophils, and influences of excitatory amino acids on cerebellar cyclic GMP. Its enzymatic formation from arginine by a soluble enzyme associated with stoichiometric production of citrulline...

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