All Photos(2)

P1093

Sigma-Aldrich

Monoclonal Anti-Paxillin antibody produced in mouse

clone PXC-10, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PXC-10, monoclonal

mol wt

antigen 68-40 kDa

contains

15 mM sodium azide

species reactivity

chicken, human, hamster, bovine, rat

application(s)

immunocytochemistry: suitable
microarray: suitable
western blot: 1:500 using a cultured human cell line extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PXN(5829)
rat ... Pxn(360820)

General description

Monoclonal Anti-Paxillin (mouse IgG1 isotype) is derived from the PXC-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Paxillin (68 kDa), is a cytoskeletal component. It is found in the ends of actin stress fibers of the focal adhesions, but not in adherens junctions of the cells. The paxillin molecule has a single binding site for vinculin, and at least two binding sites for focal adhesion kinase (FAK), that are separated by an intervening sequence of 100 amino acids.

Specificity

Recognizes an epitope located in the LIM1 domain of the paxillin molecule.

Immunogen

C-terminal part of recombinant chicken paxillin (amino acids 305-559)

Application

Monoclonal Anti-Paxillin antibody produced in mouse has been used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

Paxillin interacts with several proteins including members of the src family of tyrosine kinases, the transforming protein v-crk, the cytoskeletal protein vinculin, and the tyrosine kinase, focal adhesion kinase (FAK). This interaction has suggested a function for paxillin as a molecular adaptor, responsible for the recruitment of structural and signaling molecules to focal adhesions.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Paxillin: a new vinculin-binding protein present in focal adhesions.
Turner CE, et al.
The Journal of cell biology, 111(3), 1059-1068 (1990)
Paxillin: a crossroad in pathological cell migration
Lopez CAM, et al.
Journal of Hematology & Oncology, 10(1), 50-50 (2017)
Jung Yul Lim et al.
Biomaterials, 28(10), 1787-1797 (2007-01-16)
An important consideration in developing physical biomimetic cell-stimulating cues is that the in vivo extracellular milieu includes nanoscale topographic interfaces. We investigated nanoscale topography regulation of cell functions using human fetal osteoblastic (hFOB) cell culture on poly(l-lactic acid) and polystyrene...
Gokhan Bahcecioglu et al.
International journal of biological macromolecules, 122, 1152-1162 (2018-09-16)
In this study, porcine fibrochondrocyte-seeded agarose, methacrylated gelatin (GelMA), methacrylated hyaluronic acid (MeHA) and GelMA-MeHA blend hydrogels, and 3D printed PCL scaffolds were tested under dynamic compression for potential meniscal regeneration in vitro. Cell-carrying hydrogels produced higher levels of extracellular...
Vanessa Liévin-Le Moal et al.
Cellular microbiology, 13(7), 992-1013 (2011-04-20)
The secreted autotransporter toxin, Sat, which belongs to the subfamily of serine protease autotransporters of Enterobacteriaceae, acts as a virulence factor in extraintestinal and intestinal pathogenic strains of Escherichia coli. We observed that HeLa cells exposed to the cell-free culture...

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