P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

EC Number:
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

form

DMSO solution

shipped in

dry ice

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Packaging

1 mL in glass bottle

Application

After 48 hours in medium, add fresh inhibitors in fresh medium.

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

RIDADR

NA 1993 / PGIII

WGK Germany

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis
Certificate of Origin
Matthew Townsend et al.
The Journal of physiology, 572(Pt 2), 477-492 (2006-02-14)
The accumulation of amyloid beta-protein (Abeta) in brain regions serving memory and cognition is a central pathogenic feature of Alzheimer's disease (AD). We have shown that small soluble oligomers of human Abeta that are naturally secreted by cultured cells inhibit...
Takuma Shiratori et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 218-228 (2017-11-17)
As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the...
Asja Guzman et al.
Biomaterials, 115, 19-29 (2016-11-24)
Invasive breast cancer and other tumors of epithelial origin must breach a layer of basement membrane (BM) that surrounds the primary tumor before invading into the adjacent extracellular matrix. To analyze invasive strategies of breast cancer cells during BM breaching...
Rachel Kaminsky et al.
Developmental cell, 17(5), 724-735 (2009-11-20)
Sumoylation is a reversible posttranslational modification that plays roles in many processes, including transcriptional regulation, cell division, chromosome integrity, and DNA damage response. Using a proteomics approach, we identified approximately 250 candidate targets of sumoylation in C. elegans. One such...
Yu Li et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-02)
Dairy cows usually experience negative energy balance coupled with an increased incidence of fatty liver during the periparturient period. The purpose of this study was to investigate the effect of hepatic steatosis on the expression of the sirtuin 1 (SIRT1)...
Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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