P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

NACRES:
NA.52
Pricing and availability is not currently available.

grade

for molecular biology

pH range

4.3 - 4.7

storage temp.

2-8°C

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step.

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used for the extraction and purification of RNA.

Packaging

100, 400 mL in glass bottle

Signal Word

Danger

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

T

Risk Statement

63-23/24/25-34-40-48/20/21/22-68

Safety Statement

26-36/37/39-45

RIDADR

UN 2821 6.1 / PGII

WGK Germany

WGK 3

Flash Point(F)

175.0 °F - closed cup

Flash Point(C)

79.44 °C - closed cup

Certificate of Analysis
Certificate of Origin
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C Puissant et al.
BioTechniques, 8(2), 148-149 (1990-02-01)
C Pistore et al.
Oncogene, 36(40), 5551-5566 (2017-06-06)
Widespread genome hypo-methylation and promoter hyper-methylation of epithelium-specific genes are hallmarks of stable epithelial-to-mesenchymal transition (EMT), which in prostate cancer (PCa) correlates with castration resistance, cancer stem cells generation, chemoresistance and worst prognosis. Exploiting our consolidated 'ex-vivo' system, we show...
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory...
Protocols
Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol from Cold Spring Harbor Laboratory Press.
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