Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Pricing and availability is not currently available.


for molecular biology

pH range

4.3 - 4.7

storage temp.


General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step.


Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used for the extraction and purification of RNA.


100, 400 mL in glass bottle

Signal Word


Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes


Risk Statement


Safety Statement



UN 2821 6.1 / PGII

WGK Germany


Flash Point(F)

175.0 °F - closed cup

Flash Point(C)

79.44 °C - closed cup

Certificate of Analysis
Certificate of Origin
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C Puissant et al.
BioTechniques, 8(2), 148-149 (1990-02-01)
C Pistore et al.
Oncogene, 36(40), 5551-5566 (2017-06-06)
Widespread genome hypo-methylation and promoter hyper-methylation of epithelium-specific genes are hallmarks of stable epithelial-to-mesenchymal transition (EMT), which in prostate cancer (PCa) correlates with castration resistance, cancer stem cells generation, chemoresistance and worst prognosis. Exploiting our consolidated 'ex-vivo' system, we show...
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory...
Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol from Cold Spring Harbor Laboratory Press.
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