P2714

Sigma-Aldrich

Protease Inhibitor Cocktail powder

for general use, lyophilized powder

NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

bovine lung

form

lyophilized powder

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, and metallo-proteases.

Packaging

1 bottle in serum bottle

Application

Optimized and tested for general use.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Caution

The lyophilized powder is stable for 4 years when stored at -20°C.

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?

    One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

  2. How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?

    You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.  Aliquots can be stored at -20 C for 1 month.

  3. How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum? 

    Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.

  4. Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?

    Yes, because it is water based.

  5. Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?

    No because it contains EDTA.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. What is the difference between Protease Inhibitors S8820 and P2714?

    S8820 is the tablet version of P2714.  

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