P5542

Sigma-Aldrich

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

Quality Level

form

lyophilized powder

specific activity

≥1,000 units/mg protein (Lowry)

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

storage temp.

−20°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

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General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Packaging

5, 25 units in glass bottle

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily...
Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
D B Rawool et al.
International journal of food microbiology, 113(2), 201-207 (2006-09-19)
Clinical samples (n=725) were collected from bovines (n=243) which were positive for mastitis using the California mastitis test (CMT) and somatic cell count (SCC). The clinical samples comprising blood (n=239), milk (n=243), and faecal swabs (n=243) were examined for the...
Articles
GPI Anchored Glycoproteins
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