P8215

Sigma-Aldrich

Protease Inhibitor Cocktail

for use with fungal and yeast extracts, DMSO solution

EC Number:
MDL number:
Pricing and availability is not currently available.

Quality Level

form

DMSO solution

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Packaging

1, 5 mL in glass bottle

Application

Optimized and tested for use on fungal and yeast samples. Specifically tested on Saccharomyces cerevisiae cells.

Components

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Physical form

Solution in DMSO

Risk Statement

36/38

Safety Statement

24-26

RIDADR

NA 1993 / PGIII

WGK Germany

WGK 1

Certificate of Analysis
Certificate of Origin
Takashi Kubota et al.
Molecular & cellular proteomics : MCP, 10(7), M110-M110 (2011-04-21)
Yeast cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability. To understand the in vivo function of the Ctf18 complex, we analyzed chromatin composition in a ctf18Δ mutant using the...
Takashi Kubota et al.
Molecular cell, 50(2), 273-280 (2013-03-19)
The ring-shaped complex PCNA coordinates DNA replication, encircling DNA to act as a polymerase clamp and a sliding platform to recruit other replication proteins. PCNA is loaded onto DNA by replication factor C, but it has been unknown how PCNA...
Jin H Lee et al.
Virology, 378(2), 347-354 (2008-07-08)
In this study we have defined protein-protein interactions between the structural proteins of herpes simplex virus type 1 (HSV-1) using a LexA yeast two-hybrid system. The majority of the capsid, tegument and envelope proteins of HSV-1 were screened in a...
Wei Xie et al.
Molecular biology of the cell, 20(14), 3317-3329 (2009-05-22)
Endoplasmic reticulum (ER) quality control mechanisms monitor the folding of nascent polypeptides of the secretory pathway. These are dynamic processes that retain folding proteins, promote the transport of conformationally mature proteins, and target misfolded proteins to ER-associated degradation (ERAD) pathways....
Carol S Bookwalter et al.
Molecular biology of the cell, 20(14), 3414-3421 (2009-05-30)
Myo4p, a single-headed and nonprocessive class V myosin in budding yeast, transports >20 different mRNAs asymmetrically to the bud. Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter...

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