Pyridoxal 5′-phosphate hydrate


3-Hydroxy-2-methyl-5-([phosphonooxy]methyl)-4-pyridinecarboxaldehyde, Pyridoxal phosphate, PLP, Pyridoxal 5-phosphate, Codecarboxylase
Empirical Formula (Hill Notation):
C8H10NO6P · xH2O
CAS Number:
Molecular Weight:
247.14 (anhydrous basis)
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source

synthetic (organic)






HPLC: suitable


off-white to yellow


140-143 °C

storage temp.


SMILES string




InChI key


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General description

Pyridoxal 5′-phosphate (PLP) is synthesized in a multiple-step process. The two pathways inlcude pyridoxal phosphate biosynthetic protein (PdxA)- pyridoxine-5′-phosphate synthase (PdxJ) pathway and the pyridoxal 5′-phosphate synthase subunit PDX1/PDX2 pathway. It is the active form of pyridoxine.


Pyridoxal 5′-phosphate hydrate has also been used:
  • as a reference standard to quantify vitamin B6 in feed and digesta samples using high performance liquid chromatography (HPLC)
  • in D-amino acid transaminase reaction(10)
  • as a cofactor for L-glutamic acid decarboxylase


1, 5, 25 g in poly bottle

Biochem/physiol Actions

Pyridoxal 5′-phosphate (PLP) aids in carbohydrate and fat metabolism by serving as a cofactor. It is majorly responsible for catalyzing the enzymatic reactions involved in sphingolipid synthesis and neurotransmitter (dopamine and serotonin) synthesis. PLP is used in the studies of PLP-dependent enzyme active sites. PLP is also a cofactor for a wide range of enzymes including mitochondrial 5-Aminolevulinic acid synthase (ALAS) cysteine desulfurase, cystathionine γ-synthase (CGS), ornithine 4,5-aminomutase (OAM), and D-serine dehydratase.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Physical and enzymological interaction of Bacillus subtilis proteins required for de novo pyridoxal 5?-phosphate biosynthesis
Belitsky BR
Journal of Bacteriology, 186(4), 1191-1196 (2004)
Structure of the cystathionine gamma-synthase MetB from Mycobacterium ulcerans
Clifton MC, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 67(9), 1154-1158 (2011)
Preparation, Stimulation and Other Uses of Adult Rat Brain Synaptosomes
Modi J, et al.
Bio-protocol, 7(24) (2017)
Structure of the mitochondrial aminolevulinic acid synthase, a key heme biosynthetic enzyme
Brown BL, et al.
Structure, 26(4), 580-589 (2018)
A catalytic mechanism that explains a low catalytic activity of serine dehydratase like-1 from human cancer cells: crystal structure and site-directed mutagenesis studies
Yamada T, et al.
Biochim. Biophys. Acta Gen. Subj., 1780(5), 809-818 (2008)

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