P9541

Sigma-Aldrich

Potassium chloride

for molecular biology, ≥99.0%

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

Quality Level

grade

for molecular biology

assay

≥99.0%

mp

770 °C (lit.)

density

1.98 g/mL at 25 °C (lit.)

cation traces

Fe: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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General description

Potassium chloride (KCl) is useful in studies of ion transport and potassium channels. KCl is known to be used in the crystallization of histone core octamers.

Application

Potassium chloride has been used:
  • as a component of Danieau medium and also used in the preparation of phosphate buffer saline
  • in the preparation of electrolyte solution for electrochemical impedance spectroscopy and cyclic voltammetry measurements
  • as a component of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)-buffered Tyrode′s solution

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Packaging

500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Fluid osmolarity acutely and differentially modulates lymphatic vessels intrinsic contractions and lymph flow
Solari E, et al.
Frontiers in Physiology, 9(1), 871-871 (2018)
Reconstitution of nucleosome core particles from recombinant histones and DNA
Test, 375(20), 23-44 (2003)
Electrochemical impedance spectroscopic technique with a functionalized microwire sensor for rapid detection of foodbornepathogens
Lu L, et al.
Biosensors And Bioelectronics, 42(1), 492-495 (2013)
Diffusion through channel derivatives of the Escherichia coli FhuA transport protein
Braun M, et al.
European Journal of Biochemistry, 269(20), 4948-4959 (2002)
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)
Protocols
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Read More
<i>In Situ</i> Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
Read More

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