PD1

Sigma-Aldrich

PeroxiDetect Kit

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

storage temp.

2-8°C

General description

The PeroxiDetect Kit For the Determination of Aqueous and Lipid Hydroperoxides was developed for the measurement of peroxides in biological systems, which is an important factor in determining the degree of free radicals present in specific tissues.

Application

For the determination of aqueous peroxides and lipid hydroperoxides. Peroxides serve as a source for hydroxyl or peroxyl reactive radicals. These radicals can interact with DNA, proteins or lipid components of cells, causing cell damage that could lead to cell death. The measurement of peroxides in biological systems is an important factor in determining the degree of free radicals present in specific tissues. Lipid peroxidation has been proposed to contribute to various pathophysiological cell and tissue abnormalities. For example, increased levels of lipid peroxidation products in red blood cells were found to correlate well with the onset of diabetes mellitus in pregnant women. Cholesterol hydroperoxides accumulate in patients having excessive blood alcohol. Measurement of hydrogen peroxide in tissues has been used to study several aspects of free radical damage such as skin aging as induced by UV light and the effect of H2O2 as an inducer of elevated tyrosinase levels in melanoma cells. H2O2 has been shown to be a potent mitogen for growth-arrested cultured human aortic smooth muscle cells. The PeroxiDetect kit is capable of detection of H2O2 in aqueous solutions in the range of 1-7 nanomoles per reaction volume or of lipid hydroperoxides in organic solvents in the range of 1-16 nanomoles per reaction volume. This kit is based on the fact that peroxides will convert Fe2+ ion to Fe3+ ion at acidic pH. The Fe3+ ion will form a colored adduct with xylenol orange which is observed at 560 nm.
PeroxiDetect Kit is suitable for use:
  • in the quantification of hydroperoxides in synaptosomal preparation
  • in measuring lipid peroxidation in melanoma cells
  • to quantify peroxides in the cerebral cortex
  • intestinal lumen sample

Peroxides will convert Fe2+ to Fe3+ ions under acidic conditions. Fe3+ ions will then form a colored adduct with xylenol orange, which is observed at 560 nm.

Components

Kit contains reagents for 100 tests of aqueous peroxide and 100 tests of organic peroxide.

Legal Information

PeroxiDetect is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • tert-Butyl hydroperoxide 1 mL

  • Hydrogen peroxide 1 mL

Signal Word

Danger

Hazard Codes

O,T

Risk Statement

7-10-21/22-23-35

Safety Statement

3/7-14-26-36/37/39-45

RIDADR

UN 3316 9

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
J Adachi et al.
Alcoholism, clinical and experimental research, 23(4 Suppl), 96S-100S (1999-05-11)
Evidence for the presence of 5alpha-hydroperoxycholest-6-en-3beta-ol (cholesterol 5alpha-hydroperoxide, Ch 5alpha-OOH) and 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ols (cholesterol 7-hydroperoxides: Ch 7alpha-OOH and Ch 7beta-OOH, respectively) in human erythrocyte membrane was found. Blood samples were collected from alcoholic patients and healthy volunteers (controls), and...
H Masaki et al.
Biochemical and biophysical research communications, 206(2), 474-479 (1995-01-17)
The purpose of this study was to identify the active oxygen species generated in murine skin fibroblasts under UVB irradiation. When fibroblasts were exposed to UV light (UVA + UVB), hydroxyl radicals were detectable by ESR-spin trapping using DMPO as...
Formation of malondialdehyde (MDA), 4-hydroxy-2-hexenal (HHE) and 4-hydroxy-2-nonenal (HNE) in fish and fish oil during dynamic gastrointestinal in vitro digestion
Larsson K, et al.
Food & Function, 7(2), 1176-1187 (2016)
E Karg et al.
The Journal of investigative dermatology, 100(2 Suppl), 209S-213S (1993-02-01)
The effects of systems generating active oxygen species (superoxide anion, hydrogen peroxide, hydroxyl radical) on tyrosinase have been studied in cultured human melanoma cells. Tyrosinase activity was determined by measuring the quantity of 5-S-L-cysteinyl-L-dopa (5-S-CD) formed in the presence of...
D Carone et al.
European journal of obstetrics, gynecology, and reproductive biology, 51(2), 103-109 (1993-10-01)
Oxygen free radicals produced during normal aerobic metabolism have been implicated in several pathophysiological mammalian processes. The importance of free radical-mediated fatty acid oxidation has received much attention. The generation of active oxygen species may lead to lipid peroxidation and...

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