phospho-Insulin Receptor β Subunit (pTyr1158) ELISA

β Subunit (pTyr1158) phospho, sufficient for 96 tests

Insulin Receptor β Subunit ELISA, IR β Subunit ELISA
Pricing and availability is not currently available.


sufficient for 96 tests

shipped in

wet ice

storage temp.


Gene Information

human ... INSR(3643)

General description

A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal capture antibody specific for the insulin receptor (IR) β subtype (regardless of phosphorylation state) has been coated onto the multiwell strips provided with the kit. Standard dilutions and samples are incubated for 2 hours at RT. IR antigen binds to the capture antibody. After a wash, a detection antibody specific for the non-phosphorylated or phosphorylated protein is incubated for 1 hr at RT, which results in binding to the immobilized IR protein. An anti-rabbit IgG-HRP binds to the immobilized protein, completing the four-member sandwich. The reaction is visualized by tetramethylbenzidine (TMB) substrate, followed by the stop solution. The intensity of the yellow color, measured in a multiwell plate reader at 450 nm, is directly proportional to the concentration of IR in the original sample. The unknown concentrations are calculated from the standard curve run with each assay.


phospho-Insulin Receptor β Subunit (pTyr1158) ELISA is specific for native and recombinant IR that is phosphorylated at tyrosine 1158.
The assay is designed for the quantitative determination of human, mouse, and rat IR in cell lysates.

Biochem/physiol Actions

Insulin receptor (IR) belongs to the superfamily of the growth factor receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. Once activated, the IR initiates a variety of metabolic functions including glucose transport, glycogen synthesis, protein synthesis, translational control, and mitogenesis.

Features and Benefits

The IR ELISAs offer a fast (total time 4 hours), sensitive, and specific alternative to immunoblotting and other bioassays.

Analysis Note

The sensitivity is <0.8 units/mL, which is 2x more sensitive than immunoblotting. Peptide competition shows that only the phosphopeptide corresponding to the region surrounding tyrosine 1158 blocks the ELISA signal.


UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
F P Ottensmeyer et al.
Biochemistry, 39(40), 12103-12112 (2000-10-04)
Transmembrane signaling via receptor tyrosine kinases generally requires oligomerization of receptor monomers, with the formation of ligand-induced dimers or higher multimers of the extracellular domains of the receptors. Such formations are expected to juxtapose the intracellular kinase domains at the...

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