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PKH2GL

Sigma-Aldrich

PKH2 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

EC Number:

form

liquid

storage condition

protect from light

application(s)

cell analysis: suitable
detection: suitable
flow cytometry: suitable

fluorescence

λex 490 nm; λem 504 nm (PKH2)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Application

This kit is for general cell membrane labeling. It has been characterized in a number of model systems and has been found to be useful for in vitro cell labeling, in vitro proliferation studies and short term, in vivo cell tracking. The half-life for elution of PKH2 from labeled rabbit red blood cells is 10-11 days. This slow elution may complicate interpretation of experiments assessing in vivo proliferation.

Linkage

For additional technical details on PKH Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit here.

Kit Components Only

Product No.
Description

  • Diluent A 6 x 10

  • PKH2 Cell Linker in ethanol .5 mL

Certificate of Analysis

Certificate of Origin

A D Michelson et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(21), 11877-11882 (1996-10-15)
To examine the hypothesis that surface P-selectin-positive (degranulated) platelets are rapidly cleared from the circulation, we developed novel methods for tracking of platelets and measurement of platelet function in vivo. Washed platelets prepared from nonhuman primates (baboons) were labeled with...
D Flieger et al.
Journal of immunological methods, 180(1), 1-13 (1995-03-13)
Monocyte/macrophage-mediated cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) are slow processes, requiring cocultivation of effector and target cells for up to several days. Because of the high spontaneous release and possible reutilization of isotopic labels, the conventional radioactive release assays are...
Yuan Sun et al.
Cell transplantation, 20(2), 233-243 (2010-08-20)
Femoral head osteonecrosis (ON) is a serious complication of steroid administration. We examined whether implantation of autologous bone marrow-derived endothelial progenitor cells (EPCs) can augment neovascularization and bone regeneration in steroid-induced osteonecrosis of the femoral head. Forty 12-week-old male New-Zealand...
Paul K Wallace et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(11), 1019-1034 (2008-09-13)
Cell-tracking reagents such as the green-fluorescent protein labeling dye CFSE and the red-fluorescent lipophilic membrane dye PKH26 are commonly used to monitor cell proliferation by flow cytometry in heterogeneous cell populations responding to immune stimuli. Both reagents stain cells with...
Dmitry O Traktuev et al.
Circulation research, 102(1), 77-85 (2007-10-31)
It has been shown that stromal-vascular fraction isolated from adipose tissues contains an abundance of CD34+ cells. Histological analysis of adipose tissue revealed that CD34+ cells are widely distributed among adipocytes and are predominantly associated with vascular structures. The majority...

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