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PKH2PCL

Sigma-Aldrich

PKH2 Green Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

form

liquid

application(s)

cell analysis: suitable
detection: suitable
flow cytometry: suitable

fluorescence

λex 490 nm; λem 504 nm (PKH2)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Application

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for more than 21 days in vivo. Intraperitoneal or intravenous injections of the PKH2 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Linkage

For additional technical details on PKH Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit www.sigma-aldrich.com/pkh.

Legal Information

Distributed for Phanos Technologies.

Kit Components Only

Product No.
Description

  • Diluent B 6 x 10

  • PKH2 Cell Linker in ethanol .5 mL

Certificate of Analysis

Certificate of Origin

Rachel T van Beem et al.
Journal of immunology (Baltimore, Md. : 1950), 180(7), 5141-5148 (2008-03-21)
The number of colony forming unit-endothelial cells (CFU-EC) in human peripheral blood was found to be a biological marker for several vascular diseases. In this study, the heterogeneous composition of immune cells in the CFU-ECs was investigated. We confirmed that...
Fluorescent cell labeling for in vivo and in vitro cell tracking.
P K Horan et al.
Methods in cell biology, 33, 469-490 (1990-01-01)
M J Melnicoff et al.
Cellular immunology, 118(1), 178-191 (1989-01-01)
The kinetics of macrophage (M phi) recruitment to the peritoneum following the induction of acute inflammation by thioglycollate broth (TG) was evaluated after prelabeling resident M phi with the fluorescent cell tracking dye, PKH-1. Most of the PKH-1-labeled resident M...
M J Melnicoff et al.
Journal of leukocyte biology, 43(5), 387-397 (1988-05-01)
A novel method for labeling resident peritoneal macrophages (M phi) by injection of a dye into the peritoneal cavity is described. The dye, which fluoresces green, is selectively taken up by the resident M phi. Dye labeled cells can be...

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