PLN70

Sigma-Aldrich

GenElute Plasmid Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonym(s):
Gen Elute, GenElute Plasmid Kit
NACRES:
NA.52

Quality Level

usage

sufficient for 70 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

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General description

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictograms

FlameExclamation mark

Signal Word

Warning

Precautionary Statements

Target Organs

Central nervous system

RIDADR

UN 3316 9

WGK Germany

WGK 2

Certificate of Analysis
Certificate of Origin
Comparison of three molecular methods for the detection of pilchard herpesvirus in archived paraffin-embedded tissue and frozen tissue.
Crockford M
Diseases of Aquatic Organisms, 82(1), 37-44 (2008)
Cloning and Characterization of Serine-rich Entamoeba histolytica Protein Gene from an Iranian E. histolytica Isolate
Sima Rasti
Pakistan journal of biological sciences: PJBS, 9, 654-658 (2006)
Genetic determinants of resistance to fusidic acid among clinical bacteremia isolates of Staphylococcus aureus.
Lannergard J
Antimicrobial Agents and Chemotherapy, 53(5), 2059-2065 (2009)
Keiji Ueno et al.
Chemistry Central journal, 5(1), 16-16 (2011-04-06)
We have previously reported on the variation of total fructooligosaccharides (FOS), total inulooligosaccharides (IOS) and inulin in the roots of burdock stored at different temperatures. During storage at 0°C, an increase of FOS as a result of the hydrolysis of...
Isabella Monne et al.
Journal of clinical microbiology, 46(5), 1769-1773 (2008-03-28)
Among the different hemagglutinin (HA) subtypes of avian influenza (AI) viruses, H5, H7, and H9 are of major interest because of the serious consequences for the poultry industry and the increasing frequency of direct transmission of these viruses to humans....
Protocols
The product bulletin providin detailed use protocol for easy DNA transfection.
Read More
Product manual provides detailed protocol for easy DNA transfection.
Read More

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