A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).
p38 MAP kinase (MAPK), also known as a CDC-2-related protein kinase or CSBP (cytokine suppressive anti-inflammatory drug binding protein) regulates many cellular processes including inflammation, cell differentiation, cell growth and death. p38 MAP kinase is the human homolog of the S. cerevisiae gene Hog1, which is a MAPK required for growth under high-osmolarity conditions. The kit is designed to detect and quantify the levels of phosphorylated and non-phosphorylated p38 MAPK from cell lysates. It detects native and recombinant p38 protein.
For research use only. Not for use in diagnostic procedures.
p38 MAPK ELISA specifically measures human, monkey, and mouse p38 MAPK.
Features and Benefits
The assays are a sensitive, quantitative, and economical alternative to traditional immunoblotting or bioassays. They are fast (4 hours), easy to perform (precoated plates, room temperature incubations, no proprietary equipment required) and the results are reproducible from lot-to-lot.
The sensitivity is <16 pg/mL of human p38 MAPK, which is 10x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.