The ProteoQwest Colorimetric Western Blotting Kit, TMB Substrate, is designed for high sensitivity, low background colorimetric detection of as little as 0.25 ng of protein. The colorimetric reaction occurs directly on the PVDF or nitrocellulose membrane; no darkroom or film is needed. All of the components of this ProteoQwest kit have been extensively tested and optimized. This kit is designed for 25 mini-gel sized blots. It is possible to use this kit for as many as 45 blots if half the suggested amount of reagents is used.
Features and Benefits
- Short procedure time
- Reproducible results
- Minimal background
Store the reagents as supplied in the kit at 2-8 °C, except for the Chemichrome Ultimate, which should be stored in the freezer at −20 °C. The unreconstituted packets and reagents are stable for one year. Once reconstituted, the Tris buffered saline with Tween 20 is stable for 2 weeks at 2- 8 °C, if filtered into a sterile container using 0.2 μm sterile filter. Once reconstituted, the Tris buffered saline with 3% milk is stable for 1 day at 2-8 °C.
ProteoQwest is a trademark of Sigma-Aldrich Co. LLC