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PTP101

Sigma-Aldrich

Protein Tyrosine Phosphatase (PTP) Assay Kit, Non-Radioactive

sufficient for 96 reactions

Pricing and availability is not currently available.

usage

sufficient for 96 reactions

shipped in

wet ice

storage temp.

2-8°C

Analysis Note

Detection and characterization of protein tyrosine phosphatase (PTP) activity during purification (column fractions, etc.) of natural and recombinant PTPases, such as LAR phosphatase (CD45), in cell, tissue extracts and immunoprecipitates. The kit rapidly detects the inorganic phosphate released by PTPase from synthetic phosphotyrosine peptide substrates based on a colorimetric assay using malachite green-molybdate complex that shifts in light absorbance to 620-630 nm in the presence of inorganic phosphates.

Footnote

Phosphotyrosine substrates supplied are sufficient for 120 reactions.

Kit Components Only

Product No.
Description

  • Dye Additive Solution 1 mL

  • Malachite Green-Molybdate Dye Solution 1 x 25

  • Phosphate Standard (10 mM) 1 mL

  • Phosphotyrosine Peptide Substrate 2, DADEpYLIPQQG 1.6 mg

  • Phosphotyrosine Peptide Substrate 1, TRDIpYETDYYRK 1.7 mg

  • Sephadex G-25 Prepacked Column (3 ml) 5 ea

  • Water, phosphate-free 25 mL

  • 96 Well Microtiter Plate A1/2 area with cover 1 ea

See All (8)

RIDADR

UN 1789 9/PG 3

Certificate of Analysis
Certificate of Origin

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