R1258

Sigma-Aldrich

Kpn I from Klebsiella pneumoniae

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-GGTAC/C-3′
Cutting results: a 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.

Application

KpnI is a DNA restriction enzyme that is used in molecular biology research to cleave DNA at the recognition sequence 5′-GGTAC/C-3′ to generate DNA fragments with 3′-cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SL (B3782)
Comment: Kpn I shows abnormal DNA cleaving patterns (star activity) in reactions containing high (>5%) glycerol, >5% (v/v); excess enzyme and high pH (>8.0).
The enzyme will not cleave DNA containing 3′-residue of 5-methylcytosine.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4°C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
T Welsch et al.
American journal of physiology. Renal physiology, 281(4), F769-F777 (2001-09-13)
Mice lacking the 80-kDa CD2-associated protein (CD2AP) develop progressive renal failure that starts soon after birth with proteinuria and foot process effacement by unknown mechanisms. CD2AP has been identified and cloned independently by virtue of its interaction with the T...
Karel De Gendt et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(5), 1327-1332 (2004-01-28)
Androgens control spermatogenesis, but germ cells themselves do not express a functional androgen receptor (AR). Androgen regulation is thought to be mediated by Sertoli and peritubular myoid cells, but their relative roles and the mechanisms involved remain largely unknown. Using...
J Tomassini et al.
Nucleic acids research, 5(11), 4055-4064 (1978-11-01)
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by...

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