R1508

Sigma-Aldrich

Pvu I from Proteus vulgaris

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

≥5000 units/mL
5,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-CGAT/CG-3′
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Application

PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 300 mM KCl, 1 mM dithioerythritol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C.

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
T R Gingeras et al.
Nucleic acids research, 9(18), 4525-4536 (1981-09-25)
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5'...
Sanbing Shen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(43), 10893-10904 (2008-10-24)
Disrupted-in-Schizophrenia-1 (DISC1), identified by positional cloning of a balanced translocation (1;11) with the breakpoint in intron 8 of a large Scottish pedigree, is associated with a range of neuropsychiatric disorders including schizophrenia. To model this mutation in mice, we have...
E Bonnelye et al.
The Journal of cell biology, 153(5), 971-984 (2001-05-31)
The orphan nuclear estrogen receptor-related receptor alpha (ERRalpha), is expressed by many cell types, but is very highly expressed by osteoblastic cells in which it transactivates at least one osteoblast-associated gene, osteopontin. To study the putative involvement of ERRalpha in...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by...

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