R1761

Sigma-Aldrich

Cfo I from Clostridium formicoaceticum

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition Sequence: 5′-GCG/C-3′
Cutting results: a 2-10-fold Cfo I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Not complete at 65 °C for 10 minutes.

Application

CfoI is restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-GCG/C-3′ to generate restriction fragments with 3′- cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SL (B3782).

Linkage

Isoschizomer: Hha I

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol, 0.1mM PMSF, 50% glycerol (v/v), at 4 °C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
Vanja Karamatic Crew et al.
Transfusion, 47(3), 492-498 (2007-02-27)
The null phenotype of the Lutheran blood group system, Lu(null) or Lu(a-b-), is characterized by the lack of all Lutheran system antigens. It can arise from three genetic backgrounds: recessive, dominant, or X-linked. Lu(null) of the recessive type appears to...
R A Makula et al.
Nucleic acids research, 8(14), 3125-3131 (1980-07-25)
The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme...
Alessia Ongaro et al.
Haematologica, 94(10), 1391-1398 (2009-08-04)
The antifolate agent methotrexate is an important component of maintenance therapy in acute lymphoblastic leukemia, although methotrexate-related toxicity is often a reason for interruption of chemotherapy. Prediction of toxicity is difficult because of inter-individual variability susceptibility to antileukemic agents. Methotrexate...
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential...
Annabel A Ferguson et al.
Methods in molecular biology (Clifton, N.J.), 940, 87-102 (2012-10-30)
The generation of transgenic animals is an essential part of research in Caenorhabditis elegans. One technique for the generation of these animals is biolistic bombardment involving the use of DNA-coated microparticles. To facilitate the identification of transgenic animals within a...

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