R3131

Sigma-Aldrich

Bln I from Brevibacterium linens

buffered aqueous glycerol solution

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.

Application

BlnI is a restriction endonuclease that is used in molecular biological methods to cleave DNA at the recognition site 5′-C/CTAGG-3′, generating fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Caution

Enzyme in storage buffer stable at −15 to −25 °C. Store at ­-20 °C.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol (v/v) at 4°C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
M McClelland et al.
Nucleic acids research, 15(15), 5985-6005 (1987-08-11)
Fundamental to many bacterial genome mapping strategies currently under development is the need to cleave the genome into a few large DNA fragments that can be resolved by pulsed field gel electrophoresis. Identification of endonucleases that infrequently cut a genome...
Niall De Lappe et al.
BMC microbiology, 9, 155-155 (2009-08-04)
With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of...
D L Daniels
Nucleic acids research, 18(9), 2649-2651 (1990-05-11)
The complete 13 site AvrII restriction map of the genome of E coli strain MG1655 is presented and compared with several other E. coli strains. The map was determined primarily by isolating individual AvrII fragments from pulsed-field gels, and hybridizing...
Thiago Fonseca-Silva et al.
Pathobiology : journal of immunopathology, molecular and cellular biology, 79(1), 18-23 (2012-01-13)
To investigate the DNMT3B (C46359T) polymorphism and immunoexpression of DNMT3b and DNMT1 in oral lichen planus (OLP) compared to a control group. We aimed to investigate the DNMT3B (C46359T) polymorphism and immunoexpression of DNMT3b and DNMT1 in OLP (n =...
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against...

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