R4251

Sigma-Aldrich

Ribonucleic acid, transfer from Escherichia coli

Type XXI, Strain W, lyophilized powder

Synonym(s):
Transfer RNA, tRNA
CAS Number:
MDL number:
Pricing and availability is not currently available.

type

Type XXI

form

lyophilized powder

storage temp.

−20°C

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Application

Ribonucleic acid, transfer from Escherichia coli, Type XXI, Strain W may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Biochem/physiol Actions

Transfer RNA is an amino acid acceptor in the reaction with Aminoacyl-tRNA Synthetase and various amino acids, resulting in the formation of aminoacyl-tRNA.

Unit Definition

One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Premal Shah et al.
Cell, 153(7), 1589-1601 (2013-06-26)
Deep sequencing now provides detailed snapshots of ribosome occupancy on mRNAs. We leverage these data to parameterize a computational model of translation, keeping track of every ribosome, tRNA, and mRNA molecule in a yeast cell. We determine the parameter regimes...
Daniel Castro-Roa et al.
Nature chemical biology, 9(12), 811-817 (2013-10-22)
Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic...
Jean Lehmann et al.
Nucleic acids research, 41(10), 5494-5502 (2013-04-13)
The structure and function of conserved motifs constituting the apex of Stem I in T-box mRNA leaders are investigated. We point out that this apex shares striking similarities with the L1 stalk (helices 76-78) of the ribosome. A sequence and...
Stefan Klumpp et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(42), 16754-16759 (2013-10-02)
Bacterial growth is crucially dependent on protein synthesis and thus on the cellular abundance of ribosomes and related proteins. Here, we show that the slow diffusion of the bulky tRNA complexes in the crowded cytoplasm imposes a physical limit on...
Jin Chen et al.
Nature structural & molecular biology, 20(6), 718-727 (2013-04-30)
During translation elongation, the ribosome compositional factors elongation factor G (EF-G; encoded by fusA) and tRNA alternately bind to the ribosome to direct protein synthesis and regulate the conformation of the ribosome. Here, we use single-molecule fluorescence with zero-mode waveguides...

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