R4503

Sigma-Aldrich

Sma I from Serratia marcescens Sb

Restriction Enzyme

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-CCC/GGG-3′
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.

Application

SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Comment: Half-life of Sma I at 37 °C is approximately 15 minutes.

Linkage

Isoschizomer: Xma I. Unlike Xma I, Sma I produces blunt-ended fragments.

Unit Definition

One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hr. at 25 °C in a total volume of 25 ml of 1x digestion buffer SA forrestriction enzymes.

Physical form

Solution in 10 mM potassium phosphate, pH 7.0, 1 mM EDTA, 300 mM NaCl, 5 mM dithiothreitol, 50% glycerol (v/v) , 0.02% polydocanol (v/v), at 4 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

D Savoia et al.
The Journal of antimicrobial chemotherapy, 45(1), 41-47 (2000-01-11)
Two hundred and twenty one Streptococcus pyogenes isolates collected from throat swabs of untreated children with uncomplicated pharyngotonsillitis living in two centres situated in the north of Italy were tested to evaluate their macrolide resistance phenotype. Isolates were also typed...
Two restriction-like enzymes from Xanthomonas malvacearum.
S A Endow et al.
Journal of molecular biology, 112(3), 521-529 (1977-05-25)
Leila C Campos et al.
Diagnostic microbiology and infectious disease, 65(2), 112-115 (2009-08-28)
The newly described Streptococcus pneumoniae serotype 6C accounted for 2.3% (16/709) of meningitis cases and 3.2% (3/95) of nasopharyngeal isolates from healthy individuals in Brazil. The strains were multidrug resistant (18.8%) and genetically diverse. Despite low serotype 6C prevalence, continuous...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential...

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