Reference Dye for Quantitative PCR

100 ×, solution

Pricing and availability is not currently available.

Quality Level




sufficient for ≥600 reactions


100 ×



storage temp.


Related Categories


Reference Dye for Quantitative PCR has been used in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR). It has also been used as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR).
Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.


0.3 mL in poly bottle


Exclamation mark

Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of...
Ryan Manow et al.
Journal of industrial microbiology & biotechnology, 39(7), 977-985 (2012-03-01)
Previously, a native homoethanol pathway was engineered in Escherichia coli B by deletions of competing pathway genes and anaerobic expression of pyruvate dehydrogenase (PDH encoded by aceEF-lpd). The resulting ethanol pathway involves glycolysis, PDH, and alcohol dehydrogenase (AdhE). The E....
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Read More
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.