R5268

Sigma-Aldrich

Sac I from Streptomyces achromogenes

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-GAGCT/C-3′
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.

Application

SacI is a restriction enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-GAGCT/C-3′, generating DNA restriction fragments with 3′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Comment: Sac I activity is inhibited by salt concentrations above 10 mM.

Physical form

Solution in 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Restriction and modification enzymes and their recognition sequences.
R J Roberts
Nucleic acids research, 11(1), r135-r167 (1983-01-11)
Novel approach to cell sampling from preimplantation ovine embryos and its potential use in embryonic genome analysis.
Leoni, G., et al.
J. Reprod. Fert., 119, 309-309 (2000)
Lorenza Dalla Costa et al.
Journal of agricultural and food chemistry, 57(7), 2668-2677 (2009-03-07)
We have developed an effective strategy based on real-time PCR assay for the molecular characterization of genetically modified grape and to quantify the efficiency of a marker gene removal. This research has been implemented in Vitis vinifera cv. Brachetto plantlets...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against...

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