R5634

Sigma-Aldrich

Nhe I from Neisseria mucosa heidelbergensis

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-G/CTAGC-3′
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Application

NheI is a restriction endonuclease that is used in molecular biology to cleave DNA at the recognition site 5′-G/CTAGC-3′ to yield fragments with 5′-cohesive termini compatible with ends generated by AvrII, SpeI and XbaI.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Comment: High salt concentrations will inhibit activity of Nhe I.

Physical form

Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.2% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

RIDADR

UN3373 - class 6.2 Biological substance, Category B

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Restriction and modification enzymes and their recognition sequences.
Roberts, R.J.
Nucleic Acids Research, 13, r165-r165 (1995)
Carlo Bergamini et al.
Hepatology (Baltimore, Md.), 46(6), 1801-1809 (2007-10-20)
Hepatocellular carcinoma (HCC) growth severely affects prognosis. Ki-67, a known marker of cell proliferation, is a negative prognostic factor in HCC. Growth factors such as the epidermal growth factor (EGF) induce HCC cell proliferation but do not explain the great...
Amit Awasthi et al.
Journal of immunology (Baltimore, Md. : 1950), 182(10), 5904-5908 (2009-05-06)
IL-23, an IL-12 family member, has been implicated in the development of Th17 cells and the progression of autoimmune diseases. However, due to the lack of availability of sensitive Ab reagents specific for the IL-23 receptor (IL-23R), it has been...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis...

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