Ssp I from Sphaerotilus sp.

buffered aqueous glycerol solution, Restriction Enzyme

Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.


for molecular biology


buffered aqueous glycerol solution


10,000 units/mL

shipped in

wet ice

storage temp.



Recognition sequence: 5′-AAT/ATT-3′
Cutting results: a 2-10-fold Ssp I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.


SspI is a DNA restriction endonuclease used in methods in molecular biology to cleave DNA molecules at the recognition sequence 5′-AAT/ATT-3′ to generate DNA fragments with blunt ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Star activity is associated with Ssp I in reactions containing high concentrations of glycerol (>5%), enzyme or high pH >8.

Physical form

Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), 200 μg/ml BSA at 4 °C


NONH for all modes of transport

WGK Germany


Certificate of Analysis
Certificate of Origin
Rapid HPLC purification of a restriction enzyme from Sphaerotilus sp.
Warren, W., and Merion, M.
Biochromatography, 3, 225-225 (1988)
O Cordova Paz Soldan et al.
Parasitology research, 98(6), 576-581 (2006-01-25)
Intestinal parasitism was studied in children of Trujillo (Peru) to create a prevention and control program. Fecal samples of 489 children were examined. The general prevalence of intestinal parasitosis was found to be 68%. The most frequent pathogenic enteroparasites were...
Possible phytoplasma transovarial transmission in the psyllids Cacopsylla melanoneura and Cacopsylla pruni.
Tedeschi, R., et al.
Plant Pathology, 55, 18-18 (2006)
Restriction and modification enzymes and their recognition sequences.
R J Roberts
Nucleic acids research, 13 Suppl, r165-r200 (1985-01-01)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...

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