R6759

Sigma-Aldrich

Ssp I from Sphaerotilus sp.

buffered aqueous glycerol solution, Restriction Enzyme

Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-AAT/ATT-3′
Cutting results: a 2-10-fold Ssp I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Application

SspI is a DNA restriction endonuclease used in methods in molecular biology to cleave DNA molecules at the recognition sequence 5′-AAT/ATT-3′ to generate DNA fragments with blunt ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Star activity is associated with Ssp I in reactions containing high concentrations of glycerol (>5%), enzyme or high pH >8.

Physical form

Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), 200 μg/ml BSA at 4 °C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
Rapid HPLC purification of a restriction enzyme from Sphaerotilus sp.
Warren, W., and Merion, M.
Biochromatography, 3, 225-225 (1988)
O Cordova Paz Soldan et al.
Parasitology research, 98(6), 576-581 (2006-01-25)
Intestinal parasitism was studied in children of Trujillo (Peru) to create a prevention and control program. Fecal samples of 489 children were examined. The general prevalence of intestinal parasitosis was found to be 68%. The most frequent pathogenic enteroparasites were...
Possible phytoplasma transovarial transmission in the psyllids Cacopsylla melanoneura and Cacopsylla pruni.
Tedeschi, R., et al.
Plant Pathology, 55, 18-18 (2006)
Restriction and modification enzymes and their recognition sequences.
R J Roberts
Nucleic acids research, 13 Suppl, r165-r200 (1985-01-01)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.