R7644

Sigma-Aldrich

Transcript RNA Markers 0.28-6.6 kb

for RNA electrophoresis

NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

General description

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) contains 9 RNA transcripts from 281 – 6,583 bases. Three μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.

Components

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains a mixture of nine RNA transcripts, 281–6,583 bases
For optimal resolution, the recommended agarose gel concentration is 1.0%.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization.
Pinaud R, et al.
Nature Protocols, 3, 1370-1379 (2008)
Protocols
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More

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