R7763

Sigma-Aldrich

Cla I from Caryophanon latum L

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-AT/CGAT-3′
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.

Application

ClaI is a restriction enzyme that cleaves the DNA recognition sequence 5′-AT/CGAT-3′, generating DNA fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Cla I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).

Linkage

Isoschizomer: BspD I

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis
Certificate of Origin
Maria Maguire et al.
Cancer research, 68(9), 3232-3242 (2008-05-03)
MDM2 is a ubiquitin ligase that is best known for its essential function in the negative regulation of p53. In addition, MDM2 expression is associated with tumor progression in a number of common cancers, and in some cases, this has...
H Mayer et al.
Nucleic acids research, 9(19), 4833-4845 (1981-10-10)
From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates...
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by...
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a...
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential...

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