R7888

Sigma-Aldrich

ScrF I from Streptococcus cremoris

buffered aqueous glycerol solution

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Specificity

Recognition sequence: 5′-CC/NGG-3′
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Comment: ScrF I will only partially cleave DNA isolated from E. coli strains that have the dcm methylase (dcm+ strains).

Physical form

Solution in 10 mM Tris-HCl, pH 7.5 , 0.1 mM EDTA, 1 mM dithiothreitol, 50 mM KCl, 50% glycerol (v/v), 200 μg/ml BSA at 4 °C

WGK Germany

1

G F Fitzgerlad et al.
Nucleic acids research, 10(24), 8171-8179 (1982-12-20)
A novel sequence-specific endonuclease has been isolated from Streptococcus cremoris F. ScrFI recognises the sequence: (formula; see text) and cleaves as indicated by the arrow ( ). It is the first enzyme to recognise this sequence and the first endonuclease...
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the...
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target...
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis...
D S Jansson et al.
British poultry science, 53(6), 790-799 (2013-02-13)
1. Vancomycin-resistant Enterococcus faecium (VRE(fm)) has recently spread among Swedish broiler farms. The objectives were to investigate VRE(fm) persistence within barns between flocks, and to determine whether day-old chicks, feed or forklift trucks used for loading crates could be identified...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.