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  • R8013
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R8013

Sigma-Aldrich

Stu I from Streptomyces tubercidicus

buffered aqueous glycerol solution

CAS Number:
Enzyme Commission number:
MDL number:

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-AGG/CCT-3′
Ligation and recutting results: After 2-10-fold Stu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Packaging

5 vials
1000 units

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Comment: Overlapping dcm methylation will block cleavage by Stu I.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C

Certificate of Analysis

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Certificate of Origin

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