R8013

Sigma-Aldrich

Stu I from Streptomyces tubercidicus

buffered aqueous glycerol solution

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-AGG/CCT-3′
Ligation and recutting results: After 2-10-fold Stu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Comment: Overlapping dcm methylation will block cleavage by Stu I.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C

WGK Germany

1

H Shimotsu et al.
Gene, 11(3-4), 219-225 (1980-11-01)
A new sequence-specific endonuclease, StuI, produced by Streptomyces tubercidicus KCC S-0054, was identified and partially purified. StuI recognizes the hexanucleotide "palindromic" sequence (Formula: see text), and cleaves it at the middle, producing blunt ends.
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...

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