Recognition sequence: 5′-AGG/CCT-3′
Ligation and recutting results: After 2-10-fold Stu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Comment: Overlapping dcm methylation will block cleavage by Stu I.
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C