R8256

Sigma-Aldrich

Sfi I from Streptomyces fimbriatus

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting

Application

SfiI is a DNA restriction enzyme that is used in molecular biological methods to cleave DNA at the recognition site 5′-GGCC(N)4/NGGCC-3′ to generate fragments with 3′-cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SM (B3158).

Physical form

Solution in 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 5 mM MgCl2, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), 500 μg/ml BSA at 25 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
B Q Qiang et al.
Nucleic acids research, 12(11), 4507-4516 (1984-06-11)
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically...
M Van Looveren et al.
Journal of clinical microbiology, 36(10), 2828-2834 (1998-09-17)
In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and...
J M Johnson et al.
Applied and environmental microbiology, 61(7), 2806-2808 (1995-07-01)
Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between...
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the...
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against...

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