RTN10

Sigma-Aldrich

GenElute Mammalian Total RNA Miniprep Kit

greener alternative

sufficient for 10 purifications

Synonym(s):
Gen Elute, GenElute Mammalian RNA Kit
NACRES:
NA.52
Pricing and availability is not currently available.

usage

sufficient for 10 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

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General description

Sigma′s GenElute Mammalian Total RNA Miniprep Kit provides a simple and convenient way to isolate total RNA from mammalian cells and tissues. Protocols are provided for cells, tissues, and fibrous tissues. These protocols differ in their cell lysis and disruption conditions. Once the RNA is bound to the GenElute Binding Column, the purification procedure is the same for all starting materials. For fibrous tissues the kit must be used with proteinase K (Cat. No. P4850) to ensure effective cell disruption. The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for cesium chloride gradients, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform. Cells or tissues are lysed and homogenized in a buffer containing guanidine thiocyanate to ensure thorough
denaturation of macromolecules and inactivation of RNases. Addition of ethanol causes RNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove contaminants, RNA is eluted in 50-100 μL of Elution Solution. Up to 150 μg of total RNA can be isolated in less than 30 minutes.

If all traces of DNA contamination must be eliminated, further treatment with DNase I is recommended. DNase I digestion can be performed while the RNA is bound to the GenElute Binding Column using the On-Column DNase I Digestion Set (DNASE10 and DNASE70). Alternatively, for more stringent removal of contaminating DNA, the final RNA preparation can be treated with Amplification Grade DNase I (AMPD1).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute Mammalian Total RNA Miniprep Kit has been used to extract total RNA from tissues and cells.
The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications. Note that RNA shorter than 200 nucleotides in length, such as tRNA, 5S rRNA, and 5.8S rRNA, is not recovered efficiently under the conditions used with this kit.

Features and Benefits

  • Purifies total RNA from up to 107 cells or 40 mg of tissue per prep
  • Yields up to 150 μg of pure, concentrated total RNA per prep
  • Recover RNA from as few as 100 cells
  • Simple and efficient–12 to 18 preps in 30 minutes
  • Faster than gravity flow anion exchange methods
  • No cumbersome steps associated with resins and magnetic slurries
  • 40% more purifications per kit than the leading supplier

Other Notes

The GenElute Mammalian Total RNA Purification Kit combines silica-membrane technology with a convenient spin column format for a rapid bind, wash, and elute method to prepare high quality total RNA.
For additional information, please see www.sigma-aldrich.com/totalrna.

Principle

Samples are lysed and homogenized in guanidine thiocyanate and 2-mercaptoethanol to release RNA and inactivate RNases. Lysates are spun through a filtration column to remove cellular debris and shear DNA. The filtrate is then applied to a high capacity silica column to bind total RNA, followed by washing and elution. Up to 150 μg of total RNA can be recovered per prep in 100 μl of water. The purified RNA is ready for Northern blots (Fig. 1), RT-PCR (Fig. 2) and other common applications.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Target Organs

Central nervous system

Supp Hazards

EUH032

Hazard Codes

T,N

Risk Statement

10-23/24/25-32-34-43-48/22-50/53

Safety Statement

26-36/37/39-45-61

RIDADR

UN 3316 9

WGK Germany

WGK 3

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Certificate of Analysis
Certificate of Origin
Danielle M Bouchard et al.
Journal of gastrointestinal oncology, 10(5), 821-830 (2019-10-12)
Sumoylation is an important post-translational modification that involves the conjugation of the Small Ubiquitin-related Modifier (SUMO) onto target proteins. This modification is reversed through the catalytic activity of SUMO isopeptidases, known as SENPs. One of these SENPs, SENP1, was reported...
Refinement of the androgen response element based on ChIP-Seq in androgen-insensitive and androgen-responsive prostate cancer cell lines
Stephen Wilson
Scientific Reports, 6 (2016)
DNA cloning, structural analysis, SNP detection and tissue expression
profile of the IGF1 gene in Malabari and Attappady Black goats of India
THOMAS NAICY
Journal of genetics null
B Bahar et al.
Journal of animal science, 90 Suppl 4, 22-24 (2013-02-13)
Surgical removal of porcine intestinal tissue followed by an ex vivo challenge is an alternative technique of testing the anti-inflammatory effect of bioactive compounds in the intestine of live pigs. We investigated the effects of ex vivo incubation of porcine...
Martijn A Langereis et al.
Nucleic acids research, 42(4), 2473-2482 (2013-11-19)
Picornaviruses constitute a large group of viruses comprising medically and economically important pathogens such as poliovirus, coxsackievirus, rhinovirus, enterovirus 71 and foot-and-mouth disease virus. A unique characteristic of these viruses is the use of a viral peptide (VPg) as primer...
Protocols
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More

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