S5049

Sigma-Aldrich

Monoclonal Anti-S100A6 antibody produced in mouse

clone CACY-100, ascites fluid

Synonym(s):
Anti-Calcyclin
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CACY-100, monoclonal

contains

15 mM sodium azide

species reactivity

goat, human, bovine, canine, rat, lizard, pig

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable using native preparations
indirect immunofluorescence: 1:4,000 using bovine tongue frozen sections
western blot: suitable using denatured-reduced preparations

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... S100A6(6277)
rat ... S100a6(85247)

General description

Monoclonal Anti-S100A6 (Calcyclin) (mouse IgG1 isotype) is derived from the CACY-100 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. S100 calcium-binding protein A6 is mapped to human chromosome 1q21.3. S100A6 has been shown to possess 43-47% homology with S-100α, S-100β, and S100A2 (S100L).

Specificity

Recognizes an epitope located on S100A6 (Calcyclin in the old terminology) in a Ca2+ ion-dependent manner. The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium-binding protein, S100A2 (S100L), caltropin, the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b).

Immunogen

Ca2+-binding proteins from pig stomach tissue

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

S-100 proteins are calcium-modulated proteins that bind calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S100A6 along with other S100 family proteins bindsprotein phosphatase 5(PP5) in a calcium-dependent manner.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

I Rehman et al.
British journal of cancer, 91(4), 739-744 (2004-07-29)
S100A6 (Calcyclin) is a calcium-binding protein that has been implicated in a variety of biological functions as well as tumorigenesis. The aim of our study was to investigate the involvement of S100A6 during prostate cancer development and progression. Using immunohistochemistry...
Steven M Schlichtemeier et al.
The Journal of surgical research, 238, 127-136 (2019-02-17)
Hepatocellular carcinoma (HCC) is a common cause of cancer death worldwide. Resection offers the best chance of long-term survival, but a consistent adverse prognostic factor is the presence of microvascular invasion (MVI). In this study, surface-enhanced laser desorption/ionization time-of-flight mass...
Binding of transition metals to S100 proteins
Gilston BA, et al.
Science China: Life Sciences, 59(8), 792-801 (2016)
S Keijser et al.
The British journal of ophthalmology, 90(2), 213-217 (2006-01-21)
The authors investigated the expression of S100A1, S100A6, S100B, MelanA, and CEA in conjunctival naevi, primary acquired melanosis (PAM), conjunctival melanoma, and uveal melanoma in order to assess their potential usefulness in the pathological differential diagnosis of these entities. Paraffin...
Puja K Puri et al.
Journal of cutaneous pathology, 38(1), 14-17 (2010-11-03)
Spitz nevi typically show strong diffuse staining with S100A6, whereas staining in melanomas is commonly patchy and weak. To our knowledge, S100A6 has not been studied in pigmented spindle cell nevus (PSCN), considered by many to be a variant of...

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