By immunoblotting, the antibody reacts with human SKK2 and with human SKK3b and SKK3c. These proteins are identical to SKK3 with an additional N-terminal 29 and 34 amino acid residues, respectively. The antibody may also detect bands at 14, 30, 57, 60 and 63 kDa. The antibody is not recommended for immunoprecipitation.
synthetic peptide corresponding to amino acids 300-318 of human stress-activated protein kinase kinase 2 (SAPKK2, SKK2), also termed MAP kinase kinase 3 (MEK3, MKK3).
Solution in 0.07 M Tris-glycine, pH 7.0, containing 30% glycerol and 0.035% sodium azide