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S6683

Sigma-Aldrich

Anti-SKK2 (SAPKK2, MKK3, MEK3) (300-318) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

MDL number:

antibody form

affinity isolated antibody

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 38 kDa

species reactivity

human, rat, mouse

application(s)

western blot (chemiluminescent): 1 μg/mL using human A431 and HeLa, rat L6 and mouse 3T3 cell lysates

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAP2K3(5606)
mouse ... Map2k3(26397)
rat ... Map2k3(303200)

Specificity

By immunoblotting, the antibody reacts with human SKK2 and with human SKK3b and SKK3c. These proteins are identical to SKK3 with an additional N-terminal 29 and 34 amino acid residues, respectively. The antibody may also detect bands at 14, 30, 57, 60 and 63 kDa. The antibody is not recommended for immunoprecipitation.

Immunogen

synthetic peptide corresponding to amino acids 300-318 of human stress-activated protein kinase kinase 2 (SAPKK2, SKK2), also termed MAP kinase kinase 3 (MEK3, MKK3).

Physical form

Solution in 0.07 M Tris-glycine, pH 7.0, containing 30% glycerol and 0.035% sodium azide

Certificate of Analysis

Certificate of Origin

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